Does anyone has a working protocol of sample preparation for doing NMR for G-quadruplex formation in presence of a specific Metal Ion ?

I want to do NMR for my G-quadruplex forming Oligonucleotides in presence of a specific metal ion. Our lab is new to this topic and no one in our lab has experienced about this.I have tried using...

17 March 2020 8,355 2 View

I need help to interpret my cox proportional model?

Hi There I was performing a cox proportional model for a cancer study in SPSS and I had this output where the Exp(B) was 0.0000064 and the CI 0 to 3.69E+51 ; p= 1 How would I report this Thank...

13 August 2019 5,054 3 View

How to create a bathymetry input file for MIKE 21?

Hi, I am learning MIKE 21 model and I would like to create input bathymetry file from GEBCO 30 Arc Second data for north coastal Bay of Bengal. Which one do I need to follow? Bathymetry or mesh...

24 June 2019 4,372 5 View

Why so called dark matter is outside the galaxy?

The so called dark matter is pictured out side the galaxy. What is the reason? why cant it be a part of the galaxy itself ?

03 September 2018 155 4 View

Will someone provide me typical sorts of laboratory exercises on Machine learning?

I want to develop a laboratory module for undergraduate computer science on Machine learning. The lab should consist of a few 15-20 questions ranging from simple ones to little complex ones...

05 August 2018 1,570 6 View

How to account for the time duration of polymer immersion in solvent, in the calculations for swelling ratio?

The Flory-Rehner equation does give the relation between cross-linking density and the swelling ratio. But will there arise a difference in the swelling ratio if a polymer is immersed in a solvent...

24 May 2018 9,437 3 View

Does Id/ Ig increase or decrease when GO goes to rGO?

I have been trying to understand this. A few works I came across seemed to have contradictory results. If the Id/ Ig does increase, when GO goes to rGO, does it mean that the removal of Oxygen...

22 May 2018 1,491 6 View

Experts please help to identify these plants?

Experts please help to identify these plants. images 1 and 2 are same plant.

17 December 2017 3,633 10 View

Magnetic properties of HfO2

20 June 2017 1,834 2 View

How to deposit Graphene on HfO2 with good interface quality?

20 June 2017 1,468 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

How to validate image labeling quality within dataset?

Hello, it is about creating a dataset for semantic segmentation with three classes. The problem is that one class dominates with >90% and one class is

18 February 2021 4,254 5 View