Can someone tell me what is the exact difference between APOE-knock in mice and APOE-TR mice,Whether the cell type that produce APOE is the same in each mice strain?Thank you!
Hello,
Can you provide more information about these strains?
According to this article, knock in and TR means the same, but I am not sure which APOE knock in and TR strains you are asking about.
http://www.hindawi.com/journals/ijad/2011/810981/
Apoe transgenic mice contain the human apoE in addition to their own apoE gene. The knock-in mouse is a model where the mouse apoE is exchanged against a specific human apoE genotype (for example see
http://www.ncbi.nlm.nih.gov/pubmed/10655544, the authors created an apoE4 transgenic mouse). In the later model apoE is expressed in all tissues expressing apoE regularly in mouse whereas the expression in "normal" transgenic mice depends on the used promoter. If this is an ubiquitous promoter you will find apoE in every cell, by choosing an organ specific promoter you can limit the apoE expression.
I am not sure that TR means transgenic. Looks like TR = "Targeted Replacement".
You are very welcome, Xilin. I don't know the answer but maybe they follow Taconic nomenclature when they use strains from Taconic.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Proteins