Hello Rahul,
I don't have a full grasp of your question but if it is the significance of Raman Spectroscopy you seek, I think the answer given below should come in handy:
This is a technique for detecting molecules and studying the structural transformation they undergo during a chemical reaction. In this technique, the sample of interest is irradiated with a monochromatic light (typically a laser) resulting in the polarization of the sample’s electron cloud. In this instance, some of the incident light is absorbed by the sample’s molecule, excitation to higher vibrational (phonon) states of the molecule occurs. When relaxation of the excited electron to its ground state or lower vibrational energy occurs, emission of a photon in a direction different from that of the incident light occurs in a process termed scattering. Only emitted photons that undergo inelastic scattering results in the generation Raman bands, which are used for identifying a sample
Can we differentiate the RAMAN spectra of a powder and gel of the same material?
Dear Rahul Ranjan
Raman spectroscopy falls into the group of vibrational spectroscopies, i.e., the observed bands correspond to vibrations. Therefore, Raman spectroscopy reflects the chemical structure of a molecule and also to some extent the environment in which a given functional group (vibration) exists. For example, the Raman spectra of amorphous and crystalline polyethylene are slightly different, despite the chemical structure is the same.
If the powder form and gel form of your sample differ in chemical structure or, for example, if the powder is crystalline, you can expect to see some differences (at least shifts in band position, intensity and/or bandwidth).
Regards
Josef
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