In the distant past, developing glucose sensors, ferricyanide was dried with glucose oxidase, which did not suffer from the extreme concentration. That enzyme, although a protein, may not be a good example because it is very hardy.

My simplest suggestion: try with small quantities of your proteins. You have got nothing to lose besides some time.

Hello Riaan Laubscher ,

Thank you Falk Fish and Azam Farshadinia for your reply.

In response to your question, I personally do not think that 10 mM would be too high considering that you are using a transduction method like CV. Concerning the effect on biomolecules (antigen, antibodies, proteins or else...), it should not be a problem if you are working in mM. Higher concentration like 0,1M or higher could potentially be an issue and like Falk Fish stated earlier you could probably just make a few tests to check that. Although if you are working with very expensive biomolecules this could be an issue as well...

However, I have to say that the use of a buffer is very important when conducting electrochemical experiments on biosensors since the denaturation of biomolecules could occur if the buffer that you are using do not provide the necessary conditions (pH, concentration, temperature, nature of the electrolyte ...) for their optimal activity.

Finally, if you would want to use impedance for example, increasing the redox probe concentration would drastically change the shape of the spectra, but this should not affect the biomolecules.

Best regards,

Marie B.

Marie Berthuel Thank you for your response. I am using a suitable PBS buffer to maintain the necessary conditions for proteins. I will try the higher concentration.