Some hospitals, due to cost, (especially in the under-developed and developing countries) use non-vacuum plain tubes for serum where methodology stipulates the use of vacutainer plain tubes. Can I use the hospital's non-vacuum tubes or insist samples be collected again in vacutainer tubes? What is the effect of the non-vacuum tubes on the blood/serum?
Dear Peter,
I intend to carry out ELISA for IgG and IgM antibodies detection, as well as PCR.
Thank you
Hi Nneoma
There should not be any significant difference in the serum collected into a non-vacuum plain tube compared to a vacuum plain tube, providing the blood is collected and handled appropriately. Any difference would be due to variation in the collection of the blood. There is a greater chance for variation in collection technique when non-vacuum blood tubes are used.
Regards, Rosemary
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