N.C.J Anyanwu

11 Questions 8 Answers 0 Followers

Questions related from N.C.J Anyanwu

How do I resolve these calibration errors on AB 7500 Fast Real-time PCR system?

I was trying to recalibrate our 7500 Fast Real-time PCR system after cleaning the block but I keep getting these errors: For ROI: calibration failed: (error [-106]. incomplete number of wells...

10 November 2022 7,417 0 View

Can heparin tubes be used to collect blood samples for Plasmodium falciparum microscopy?

I need to collect blood samples for microscopy of P. falciparum. Which tube is best for this? Heparin or plain vacutainer tubes? Will RBC lysis affect detection of the parasite? Thank you.

24 April 2019 8,875 4 View

How do I preserve stool samples until the required sample size needed for further analysis is obtained?

I'm currently collecting stool samples for Rotavirus ELISA assay. Please how do I preserve the stool samples until number of samples needed are complete and how long can I preserve these samples...

18 April 2019 2,317 4 View

Could anyone please help me with step-by-step guide on how to run my meta-analysis on STATA 14.2?

I want to do a meta-analysis of previous prevalence studies for 2 subspecies of an organism in a particular location and I need to calculate/derive these: 1. Confidence Interval for individual...

20 June 2018 4,785 2 View

I need help with homozygosity mapping in genetics studies?

Please I would like to know the difference between exclusion mapping and homozygosity mapping and why we do exclusion mapping in some families and homozygosity mapping in other since they give...

09 February 2018 1,518 2 View

I need help with exclusion Mapping in genetics studies?

Please I would like to know , what are the criteria for exclusion mapping in familial cases?

09 February 2018 4,755 0 View

What are the criteria for heterozygosity mapping in familial cases?

What are the criteria for heterozygosity mapping, and is it useful to use this technique for familial cases?

09 February 2018 3,854 0 View

Is it possible and/or advisable to directly detect a virus from plasma/serum without separation of host plasma DNA/viral RNA using silica columns?

I'm currently working on an RNA virus. I'd like to know if I can go straight to using qualitative PCR for viral detection using plasma/serum without viral RNA/host DNA isolation. If not, then why?

29 September 2017 4,477 3 View

What's the best protocol for isolating plasma circulating host DNA to HTLV?

How do I isolate plasma circulating DNA of host to HTLV?

25 September 2017 1,970 2 View

What is the effect of non-vacuum plain tube on blood serum as compared to vacutainer plain tubes?

Some hospitals, due to cost, (especially in the under-developed and developing countries) use non-vacuum plain tubes for serum where methodology stipulates the use of vacutainer plain tubes. Can I...

25 July 2016 558 5 View

What is the ideal tube for storing serum samples at -20 or -80 degrees (after centrifugation and separation from whole blood) as the case may be?

I am presently sampling serum from patients for further processing in 6 months or more. I would like to ascertain the ideal tube for storage of these samples to avoid contamination, leakage,...

07 July 2016 4,482 5 View