Plant transformation and biotechnology
Hi Prithwi,
The question is kind of vague. Do you mean several T-DNA cassettes in the same binary vector? I am not so sure about the meaning of "multiple insertion" (in a vector) in your question.
I have tagged Sm1021 with GFP and mCherry. GFP is tagged with gene. mCheery is the control and vice versa. GFP-gene, mCheery only mCherry-gene,GFP only I have infected Medicago with the above...
11 December 2018 5,164 0 View
I am looking for the complete plasmid sequence of pSRK having section marker Gentamicin or Kanamycin or Tetracycline. It would be nice if any one can provide me or tell me from where to have the...
04 May 2018 7,927 3 View
I am trying to clone few genes within a very low copy no (7.4Kb) plasmid. The yield is pretty low after Mimiprep from 5ml of over night culture, eluted in 30 microliter of water/TE buffer. And...
04 May 2018 7,118 2 View
I am trying to determine the ploidy level of bacteriods (Sinorhizobium meliloti from the root nodules) taking the free-living bacteria as the reference. I would like to know how to determine the...
04 May 2017 3,478 2 View
I am planning to design primer for His tag. The general format of the forward primer : CGC: Restriction site (GGTACC) :Spacer(GGAGGTACTGA): Start codon (ATG): Six Histidine (CACCATCACCATCACCAT):...
09 October 2016 5,318 10 View
Can anybody help me to analyse LC MS MS data in scaffold file ?
08 September 2015 478 0 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
31 July 2024 5,087 4 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
31 July 2024 7,921 5 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...
31 July 2024 7,860 3 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
31 July 2024 8,939 5 View
Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.
26 July 2024 1,469 1 View
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24 July 2024 1,559 1 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View