How to quantify two different fluorescent tagged bacteria (Sm1021)from root nodules of Medicago trancatula in a competition experiment?

12 December 2018 0 5K Report

I have tagged Sm1021 with GFP and mCherry. GFP is tagged with gene. mCheery is the control and vice versa.

GFP-gene, mCheery only

mCherry-gene,GFP only

I have infected Medicago with the above constructs in different ratios: 1:1,1:10,10:1.

Is there any way to quantify the fluorescence from root nodules to have an idea regarding the competitiveness of the bacteria.

Thank you.

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