Can anybody help me to analyse LC MS MS data in scaffold file ?
I have tagged Sm1021 with GFP and mCherry. GFP is tagged with gene. mCheery is the control and vice versa. GFP-gene, mCheery only mCherry-gene,GFP only I have infected Medicago with the above...
11 December 2018 5,164 0 View
I am looking for the complete plasmid sequence of pSRK having section marker Gentamicin or Kanamycin or Tetracycline. It would be nice if any one can provide me or tell me from where to have the...
04 May 2018 7,927 3 View
I am trying to clone few genes within a very low copy no (7.4Kb) plasmid. The yield is pretty low after Mimiprep from 5ml of over night culture, eluted in 30 microliter of water/TE buffer. And...
04 May 2018 7,118 2 View
I am trying to determine the ploidy level of bacteriods (Sinorhizobium meliloti from the root nodules) taking the free-living bacteria as the reference. I would like to know how to determine the...
04 May 2017 3,478 2 View
I am planning to design primer for His tag. The general format of the forward primer : CGC: Restriction site (GGTACC) :Spacer(GGAGGTACTGA): Start codon (ATG): Six Histidine (CACCATCACCATCACCAT):...
09 October 2016 5,318 10 View
Plant transformation and biotechnology
10 November 2015 3,729 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)
06 August 2024 6,610 4 View
Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?
05 August 2024 8,836 2 View
I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this
05 August 2024 7,902 3 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
How can I interpret the data gathered without solving?
03 August 2024 9,054 3 View