Prithwi Ghosh

7 Questions 4 Answers 0 Followers

Questions related from Prithwi Ghosh

How to quantify two different fluorescent tagged bacteria (Sm1021)from root nodules of Medicago trancatula in a competition experiment?

I have tagged Sm1021 with GFP and mCherry. GFP is tagged with gene. mCheery is the control and vice versa. GFP-gene, mCheery only mCherry-gene,GFP only I have infected Medicago with the above...

12 December 2018 5,135 0 View

No bands after restriction digestion purification of a low copy no plasmid ?

I am trying to clone few genes within a very low copy no (7.4Kb) plasmid. The yield is pretty low after Mimiprep from 5ml of over night culture, eluted in 30 microliter of water/TE buffer. And...

05 May 2018 7,091 2 View

Looking for complete sequence of pSRK plasmid ?

I am looking for the complete plasmid sequence of pSRK having section marker Gentamicin or Kanamycin or Tetracycline. It would be nice if any one can provide me or tell me from where to have the...

05 May 2018 7,894 3 View

How to determine ploidy by flow cytometry?

I am trying to determine the ploidy level of bacteriods (Sinorhizobium meliloti from the root nodules) taking the free-living bacteria as the reference. I would like to know how to determine the...

05 May 2017 3,457 2 View

How to design His tag primer?

I am planning to design primer for His tag. The general format of the forward primer : CGC: Restriction site (GGTACC) :Spacer(GGAGGTACTGA): Start codon (ATG): Six Histidine (CACCATCACCATCACCAT):...

10 October 2016 5,292 10 View

What is the effect of intravector multiple insertion in plant transformation vector?

Plant transformation and biotechnology

11 November 2015 3,697 1 View

Can anybody help me to analyse LC MS MS data in scaffold file ?

09 September 2015 450 0 View