Hello everybody,
I have sequenced 4 plant taxa belonging to the same genus. I sequenced the trnH-psbA and ITS markers. Could anyone give me a detailed procedure of how I could confirm the idintification of those taxa using available sequences? i.e I need the instructions of the DNA barcoding technique used for authentication of a plant species.
Thank you all
Please have a look at this link.
https://www.researchgate.net/publication/265603651_A_DNA_barcoding_approach_to_identify_plant_species_in_multiflower_honey
Article A DNA barcoding approach to identify plant species in multif...
Kindly see through below info web..
http://botany.si.edu/projects/DNAbarcode/
Dear Ahmed,
Please have a look at this useful article.
https://www.researchgate.net/publication/41435368_DNA_barcoding_A_six-question_tour_to_improve_users%27_awareness_about_the_method
Article DNA barcoding: A six-question tour to improve users' awarene...
I think using trnH-psbA as chloroplast region is not enough because it's too short to match the sequence. Therefore it needs another region, I recommend adding rbcL or matK to make the results stronger. The useful program is TaxonDNA .
Dear Ahmed Gawhari,
Thank you for comment. Sure I will amplify another marker as you recommend. I am thinking of ITS and trnLF.
Dear Ahmed,
The inter-and intraspecific variation of each barcoding region should be characterized by calculating Kimura 2-parameter (K2P) distances in TaxonDNA (Meier & al., 2006). Next, you have to determine the barcoding gaps by comparing the distributions of intraspecific and interspecific distances in each candidate. This is done by using the program TaxonDNA and graphing the distribution of the variation.
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