TIC - GCMS and FID Chromatogram; Which is the best way to quantify the ingredients in the area normalization method? why?
Thanks
Dear Senthamizh,
Try: K D Altria, Chromatographia, (35) (3-4) 177-182.
This guy is from Glaxo!
Dr John
Dear Senthamizh,
% area normalization is a technique which is not so accurate.
In this technique the sum of all peak areas in FID or TIC is assume as 100%.
After that individual peak area is calculate with this 100% area.
For example you have three peaks in your chromatograph with peak areas 10, 15 and 25.
Therefore, the sum of them is 50.
Hence, the concentration of compound A will be 20%, B will be 30% and C will be 50%.
This is known as % area normalization technique.
Regards,
Dr Tariq
Dear Dr.Tariq,
Thank you very much. You are absolutely right and I agree with you.
I am having more than 50 components in the sample and all are volatile in nature, if it is so is it possible to apply the area normalization method?
I feel the composition of three compounds is absolutely right as per your discussion 20%, 30% and 50 %. i feel nothig is going wrong in this? Only if some non volatile/ semi volatile are present then the normalization is in question? and also i agree the % area will not match perfectly with absolute % w/w
also i would like to add some more question
Also people are saying something about detector linearity? What it is? How it will affect the real composition of the material? is the detector linearity is related to the area normalistion (% area) with absolute % (w/w)?
still my question is not answered. My question is in a ideal condition what is the difference between TIC and FIC-Chromatogram for area normalisation %? I am asking this question as part of reverse engineering /chemistry? which % will be close / near to the absolute % w/w?
also i would like to say that i can not go for reference standard for all 50-100 components and do the abolute assay.
Many thanks
Another approach is that after getting the % area of the peak you can now divide it by the amount of your dried sample used.
How area normalization purity differ from applying weight factor with respect to dried basis
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