Can we use RNA-seq library for gene expression studies?

Since our RNA is less abundant, can we use the libraries prepared for RNA-seq to validate (qPCR)the results of RNA-seq.

12 November 2020 803 6 View

For primer efficiency calculation can we use positive control(in which gene is abundantly expressed) and perform the analysis?

My sample of interest is sperm RNA(less abundant and highly fragmented RNA). In that case, to determine the primer efficiency, can i use tissue samples (in which the gene is abundantly expressed)...

11 November 2020 758 3 View

What is the difference between semantic role labelling and named entity recognition?

Natural language understanding

04 May 2019 7,442 9 View

Is there a software or Webpage where I can view a 3D anatomy map of the brain?

I want to look at all the various brain regions and also the ascending and descending tracts from the brain, and make sense of the information flow.

12 March 2019 1,778 5 View

How to calculate network performance using ns3?

I'm new to this network domain. But i want to learn this area thoroughly. So I'm currently dealing this subject. I'm learning ns-3 simulator. Please help me to know how to analyze the performance...

17 September 2018 3,246 3 View

Acyliminium reactivity versus enecarbamate?

Dear researchers, i kindly ask you to help me with the following aspect: in scheme 4 (of j.org. chem, vol.66,No.23, 2001) it is shown that nucleophile attacks on the acyliminium intermediate and...

08 February 2016 1,601 3 View

I am using HEC RAS to connect various ponds and lakes in the city and perform flood inundation mapping. What is the suitable analysis?

I am using HEC RAS (Though it is a river analysis software) to an urban area to connect various ponds and lakes in the city and perform flood inundation mapping. What is the suitable...

01 February 2016 4,321 6 View

Why does K estimator fail to estimate Ka and Ks values for certain gene combinations?

12 February 2015 8,709 1 View

Can any one explain me the pixel recursion and how to calculate new disparity candidate using this?

Pixel recursion.

11 August 2014 2,495 2 View

Can anyone guide me about disparity estimation in stereo images using line-wise hybrid recursive matching algorithm?

Disparity estimation of stereo images. This involves several processing steps including pixel recursion, post processing, how to estimate disparity between two gray scale images using this algorithm.

11 August 2014 9,312 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View