What is the difference between melting temperature and annealing temperature on the one hand molecular biology ?

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How to convert a Text File to a Fasta Format File?

10 November 2018 3,936 0 View

Which came first, the German Peasants War or the English Peasants Revolt?

German Peasants War, English Peasants Revolt

05 June 2018 7,031 1 View

Why the DNA considered a genetic material ?

DNA , genetic material

04 May 2018 9,780 0 View

Which is the most credible free online IQ test?

credible free online IQ test

04 May 2018 3,605 1 View

Are there any animals that actually smile when they're happy ?

animals that actually smile when they're happy

04 May 2018 9,762 0 View

Is an offline IQ test more reliable than a online IQ test?

offline IQ , online IQ test

04 May 2018 2,624 1 View

At the age of 28, is it too late to start a PhD in the biotechnology field or other ?

the age of 28, start a PhD

03 April 2018 3,887 98 View

What is the best way to extract DNA from fungi ?

extract DNA, fungi

02 March 2018 4,916 3 View

If the bacteria multiply all their genes within 36,000 minutes, how many genes have multiply within one hour ?

gene multiply

02 March 2018 4,777 0 View

Staying in space for a long time affects DNA or gene expression ?

space , DNA, gene expression

02 March 2018 6,631 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View