Dear Sir. Concerning your issue about the difference between Ethel acetate and Methanol in extraction process. Methanol is a polar protic solvent which also can act as a nucleophile. Ethyl acetate is a polar aprotic solvent, non-nucleophilic in nature. There is a possibility that your compound might have reacted with metanol.However, if you actually ran your second TLC in metanol, your compound might have moved all the way up with the solvent front because methanol is very polar. If you use any kind of stain for TLC developing, you can easily check it. I think the following below links may help you in your analysis:

http://scialert.net/fulltext/?doi=ijbc.2017.17.22 https://www.sciencedirect.com/science/article/pii/S1018364713000700

Thanks

https://www.researchgate.net/post/Why_do_methanol_and_ethyl_acetate_give_such_different_results_when_combined_with_a_dry_sample_and_run_with_TLC

Cool wondering bro.... .

The difference in dipole constant and viscosity ..

In case of extraction, only difference is the polarity of solvents.

methanol 5.1 & ethyl acetate 4.4

ethyl acetate + fraction of methanol will always better than 100% methanol for TLC

As well viscosity and stability ...