Our feed producing company is planning to implant the methods for residue analysis in feed, eggs, cattle and poultry meat and milk. We want it to be fast and reliable results. we have to choose between HPLS and Elisa.
Fast and reliable are often in competition, so you have to choose.
Furthermore immunoassays like ELISA measure the binding of a certain type of molecule. They do not give final information on its identity.
For that reason I would choose HPLC and preferably LC-MS to be able to identify the molecule you have observed.
Major antibiotics to detect are chlorampenicol and Nitrofurans which are prohibited to use in feed and in veterinary. So we're searching for methods in feed mill to detect easily and fast.
Why do you suspect these antibiotics to occur in the feed mill? And why do you want to check for their presence? Illegal use will probably not detected via the feed mill.
Is it a legal requirement or do you want to check the suppliers of your feed ingredients?
Check your suppliers and ask for guarantees on the products the deliver.
Ask your self: why do we want/have to do this and what do we want or need to achieve by doing it. This might help a lot in making the essential decisions about your quality control system and the tools essential for such a system.
This time in our country there is no definition an regulations of feed. We try to monitor our products and our suppliers. And also we need to serve our customers also who have their own farms of poultry (layers and broilers) your right telling that's there's no need to monitor feed for antibiotic recidues but in our country there are other needs :/ for raw material our suppliers have only GMO certificate. Nothing else. Even we analyse for mycotoxins if we have some suspects.
Both methods are comparable in their effectiveness in this area (analysis of antibiotic residues). HPLC is more selective, but significantly more expensive.
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