I need to obtain gold nano particles functionalized with branched polyethylenimine. Is functionlizing same as capping?
I need to oxidize Fe2+ ions to Fe3+ ions in aqueous medium at pH 7. Is there an oxidizing agent that I can use for this without affecting Cu2+ ions? Can hydrogen peroxide be used?
10 November 2016 6,153 6 View
I am running an assay for 5'nucleotidase activity based on measurement of ammonia through Berthelot's method. I used AMP as a substrate which is cleaved by 5 nucleotidase to form adenosine....
05 June 2016 8,901 2 View
I am growing MCF-7 cells and after 4 hours of thawing and putting in a flask, the cells are still floating. I am worried because this is an adherent cell line and should not take this long to...
05 June 2016 7,181 4 View
I am running an assay for mitochondrial complex III activity using isolated mitochondria. I am using antimycin A dissolved in DMSO as an inhibitor bur the antimycin a is not inhibitory to the...
02 March 2016 1,185 4 View
I ran an array based q PCR and obtained data for fold change in genes. I followed this up by running end pint PCR followed by a gel. But the fold change in my end point PCR is not the same as as...
07 August 2015 5,875 2 View
I ran a gradient PCR and there seems to be amplification at 52 and 54 degree celcius but not at 53 degree celcius. What could this possibly mean? My primer and DNA template concentrations were the...
03 April 2015 6,611 6 View
I want to use Amplex red to detect ROS (specifically Hydrogen peroxide) in cell treated with a toxicant. Can it be used to detect intracellular H2O2 or is it only used to detect H2O2 secreted...
03 April 2014 9,384 0 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Is anyone here using the Thermo Fisher Nano Orange kit for protein quantification, and detecting the fluorescent signal on the Promega Quantus? We are struggling to make sense of the standard...
05 August 2024 8,946 3 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?
03 August 2024 4,624 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
The delta function seems produce logical contradictions when analyzed on a fundamental level. I would be curious if anyone else agrees.
31 July 2024 10,109 3 View
Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.
31 July 2024 9,140 2 View