Vasundhra Bahl

8 Questions 12 Answers 0 Followers

Questions related from Vasundhra Bahl

How can I completely oxidize Fe2+ to Fe3+ in aqueous medium without oxidizing Cu2+?

I need to oxidize Fe2+ ions to Fe3+ ions in aqueous medium at pH 7. Is there an oxidizing agent that I can use for this without affecting Cu2+ ions? Can hydrogen peroxide be used?

11 November 2016 6,096 6 View

What is the difference between capping and functionlizing a gold nanoparticle?

I need to obtain gold nano particles functionalized with branched polyethylenimine. Is functionlizing same as capping?

11 November 2016 5,861 0 View

How long doe MCF-7 cells take to attach after thawing and putting in a flask?

I am growing MCF-7 cells and after 4 hours of thawing and putting in a flask, the cells are still floating. I am worried because this is an adherent cell line and should not take this long to...

06 June 2016 7,143 4 View

What is the correct protocol and pH to run a 5' nucleotidase assay based on measurement of ammonia?

I am running an assay for 5'nucleotidase activity based on measurement of ammonia through Berthelot's method. I used AMP as a substrate which is cleaved by 5 nucleotidase to form adenosine....

06 June 2016 8,861 2 View

Why is Antimycin A not inhibiting complex III?

I am running an assay for mitochondrial complex III activity using isolated mitochondria. I am using antimycin A dissolved in DMSO as an inhibitor bur the antimycin a is not inhibitory to the...

03 March 2016 1,147 4 View

Fold change in my end point PCR (checked through a gel) is not the same as array based RT PCR. It is normal or should they match?

I ran an array based q PCR and obtained data for fold change in genes. I followed this up by running end pint PCR followed by a gel. But the fold change in my end point PCR is not the same as as...

08 August 2015 5,840 2 View

Why is there amplification at 52 and 54 degrees Celsius but not at 53 in gradient PCR?

I ran a gradient PCR and there seems to be amplification at 52 and 54 degree celcius but not at 53 degree celcius. What could this possibly mean? My primer and DNA template concentrations were the...

04 April 2015 6,569 6 View

Can Amplex Red be used to detect intracellular ROS?

I want to use Amplex red to detect ROS (specifically Hydrogen peroxide) in cell treated with a toxicant. Can it be used to detect intracellular H2O2 or is it only used to detect H2O2 secreted...

04 April 2014 9,346 0 View