In antisense technology you will have to provide antisense strand becoz sense strand is present in plant while sense strand is already in plant.. The target gene will be silence when sense and antisense paired together. In case of RNAi, both sense and antisense are provided, which generate small RNAs whch cut mRNA into pieces and thus information is prevented from translation
RNAi is a mechanism that is defined as such only in eukaryotes so far. After duplex RNA formation, targeted mRNA is degraded non-stoichiometrically by the action of different enzyme complexes.
Knock-down of a transcript by expressing its antisense transcript can lead to a number of different actions that all lead to decreased protein production or exression. Translation initiation sites and splicing sites can be masked for example. Also in prokaryotes there are RNases that target dsRNA, but degradation this way would be stoichiomatric as far as I know (one mRNA+one asRNA --> degradation)
There is some comprehensive reading on this matter:
Achenbach et al. (http://onlinelibrary.wiley.com/doi/10.1002/cbic.200300708/pdf)
All the best, Ulrike
Markus responded your question very well.
It is important to consider if your specie have homologous gene. In plants I had a silencing about 93 % using RNAi construction under the 35s promoter. However the homologous gene of my interest gene (with a identity of 80%) was silenced only by a 35%, giving a unwanted activity, and making my transgenic plant useless. So the RNAi silencing is very specific for the gene of which was designed the construction.
Thanks to all of you for your participation, but I have one more doubt related to it, that in both cases there is the formation of dsRNA, and it is the formation of dsRNA that activates the siRNA fromation in cell, then how an antisense technology is very different from RNAi technology even in case of success rate?
In the case of antisense transformation, the reverse complement RNA must first find the native RNA before hybridization but if you transform with a hairpin construct, the double strand RNA will easily form by itself. This may explain why the RNAi technology is usually more performant.
In antisense technology you will have to provide antisense strand becoz sense strand is present in plant while sense strand is already in plant.. The target gene will be silence when sense and antisense paired together. In case of RNAi, both sense and antisense are provided, which generate small RNAs whch cut mRNA into pieces and thus information is prevented from translation
In antisense technology you will have to provide antisense strand becoz sense strand is present in plant while sense strand is already in plant.. The target gene will be silence when sense and antisense paired together. In case of RNAi, both sense and antisense are provided, which generate small RNAs whch cut mRNA into pieces and thus information is prevented from translation
In antisense technology you will have to provide antisense strand becoz sense strand is present in plant while sense strand is already in plant.. The target gene will be silence when sense and antisense paired together. In case of RNAi, both sense and antisense are provided, which generate small RNAs whch cut mRNA into pieces and thus information is prevented from translation
In antisense technology you will have to provide antisense strand becoz sense strand is present in plant while sense strand is already in plant.. The target gene will be silence when sense and antisense paired together. In case of RNAi, both sense and antisense are provided, which generate small RNAs whch cut mRNA into pieces and thus information is prevented from translation
In antisense technology you will have to provide antisense strand becoz sense strand is present in plant while sense strand is already in plant.. The target gene will be silence when sense and antisense paired together. In case of RNAi, both sense and antisense are provided, which generate small RNAs whch cut mRNA into pieces and thus information is prevented from translation
In antisense technology you will have to provide antisense strand becoz sense strand is present in plant while sense strand is already in plant.. The target gene will be silence when sense and antisense paired together. In case of RNAi, both sense and antisense are provided, which generate small RNAs whch cut mRNA into pieces and thus information is prevented from translation
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