Hi everyone,
I aim to isolate MSCs from the bone marrow of the mouse. I put the bone marrow cells into the cell culture flask containing DMEM low glucose+BFGF+vit C.
I know that now I have lots of different cells inside the flask but this type of the cells that I posted the picture are growing in a line and I am so curious to know what is the type of these cells. Does anyone have an idea?
Fahad Alshehri Shabnam Shahrivari
I am not from your field but my research with AI provided following info:
Based on your description of the cells growing in a line, it's possible that they could be fibroblast-like cells. Fibroblasts are a type of connective tissue cell commonly found in culture and have a characteristic elongated or spindle-shaped morphology. They often grow in a pattern where they align with each other, appearing like cells in a line.
To accurately identify the specific type of cells you are observing, it is important to perform additional analyses such as cell staining, immunocytochemistry, or molecular markers. These techniques can help determine the cell's characteristics, expression of specific proteins, or lineage markers, which can provide insights into their identity.
If you are specifically trying to isolate mesenchymal stem cells (MSCs) from the bone marrow of a mouse, it would be beneficial to perform additional experiments and assays to confirm the presence of MSCs. MSCs have specific characteristics and markers that can be identified through techniques such as immunophenotyping or differentiation assays.
Consult with a cell biology or stem cell expert.
Good luck
I recommend to use another brand of cell culture plate and monitor the results. I guess some uncommon surface roughness is behind this event.
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