Is it possible to research about School mapping and quality enhancement of education ?

I want to research in the topic " Relationship between School Mapping and Quality enhancement in Education"

04 January 2021 1,299 3 View

Antimicrobial properties of metal oxide nanoparticles?

08 April 2020 3,013 5 View

Can anyone help me with implementing fuzzy SVM in Python?

Can anyone help me implementing fuzzy SVM in Python or any other language? I want to see if the fuzzified SVM yeilds better results than naive SVM for my dataset.

06 March 2018 5,999 3 View

How do I use one class SVM for user authentication?

How do I implement one class SVM for user authentication? I have unlabelled dataset for 100 users. In my understanding, I have to create 100 different one class svm models for all users to...

25 February 2018 2,648 4 View

Does anyone use any tool to classify NSL-KDD attributes?

I am working on NSL-KDD data set for my research work. I want to separate all 41 attributes except 42nd class attribute into four class labels like Basic, Content, Traffic and Host. It is not...

13 July 2017 4,246 7 View

How to increase the heap size in WEKA?

13 January 2017 9,232 11 View

Anybody has any ideas about the latest primers to be used for Stingless bees (Tetragonula )?

We are working on Mitochondrial DNA bar coding of stingless bees (Tetragonula). Primers we are using now did not work very well. Wondering if there is any information about the latest primers...

18 December 2016 6,510 3 View

I am looking for distributional data for Tetragonula laeviceps in South Asia and South East Asia. Any one please suggest if you have any information?

Tetragonula is the tiny little stingless bees found in Indo-malaysian and Australian region, but I am looking for its world wide distribution to model their probable distribution in certain...

20 November 2016 6,514 2 View

Is this possible to have high Anti-TPO level while FT3 ,FT4 and TSH level are in range ?

Biochemistry, Immunology, Thyroid disorder

19 August 2016 2,064 12 View

Which species of Mycobacterium tuberculosis is a late grower?

1-Only grow in LJ media after 6 week.

25 February 2014 8,183 1 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

Good day, I have not been able to amplify the plasmid Frt-hspB1. Somebody could give an advice me?

I acquired the plasmid in Addgene, reconstituted the filter paper where the plasmid comes from with 30 microliters of TE buffer pH 8, immediately afterwards perform the transformation of E.coli...

25 January 2021 2,526 3 View

Has someone used TTC agar overlay method and can help me?

I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...

22 January 2021 585 10 View

I am having trouble with cloning my gene. How can I fix this?

16 January 2021 6,566 8 View