How to compare means of continuous data with zero-inflation and skew?

I have two groups (unpaired) of plant biomass measurements and want to know whether there is a test that can be used to determine statistical significance where assumptions are not violated. The...

18 February 2021 1,258 4 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View

Are there commercially available fluorescence-tagged Influenza A virus?

30 November 2020 9,445 1 View

What tests for: 1. differences between continuous variables when grouped with different categorical ones; 2. significance of potential relationships?

Hello. I was wondering if anyone had suggestions in terms of what exploratory analysis to carry out for my dissertation with the following data. I measured N=26 participants pre and post an...

09 August 2020 5,256 5 View

Wavy looking bands In western blots?

Hello everybody, from some time, I keep having waving bands in the lower part of my gel, like the proteins are not running on a flat front. I cast my own gels and run them at 90V at the beginning...

26 May 2020 937 3 View

SURVEY - how people changed attitude toward green spaces & urban forests during the COVID19 quarantine?

https://vub.fra1.qualtrics.com/jfe/form/SV_5bSsVfI1g1VgI7j While shaking our society and turning our way of living upside down, the COVID19 pandemic is also putting the relation between nature...

15 May 2020 2,339 8 View

Direct Input of the Occupation Matrix?

I'm implementing the Occupation Matrix Control scheme (OMC) in VASP DFT+U calculations via direcct input of the Occupation Matrix. This requires an external input file (OCCMATRIX) containing the...

17 March 2020 1,921 1 View

Glycerol topology/structure for GROMOS87/Berger forcefield?

Hello all, I need the topology /structure file of the glycerol for the GROMOS87 forcefield. Is some repository available online or do someone know how can I derive them by myself (maybe some tool...

12 March 2020 8,858 2 View

How to interpret moderating effect in PLS?

I am running a Partial Least Squadre Path Modelling in R and I have a Moderating effect (explained by one scalar variable) between my exogenous x and my endogenous y. The only variable that...

25 February 2020 8,022 4 View

Recombinant protein for antibody neutralization?

I need to understand if an antibody I'm using is specific or not. The antibody is not validated. In immunoblotting, the antibody detects a prominent band at the right molecular weight and two...

29 April 2019 4,752 4 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View