What is the concentration of dichlorofluorecein (CM-H2DCF-DA) used in neonatal rat cardiomyocytes for flow cytometry experiments?

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Pradyumna Kumar Mishra

Prepare a fresh stock solution of CMH2DCFDA (5 millimoles) in DMSO and dilute it to a final concentration of 1 micromoles in 1 X PBS. Use 50 micromoles of working solution (final working concentration adjusted to 2.5 micrograms/50 microlitres).

Please see the detailed method described in our attached publication.

Good luck.