Can any suggest me good method of GC MS for the separation of Flavonoids?

I prepared the ethyl acetate, ethanol and butanol fraction from the ethanol extract of seed for isolation of Flavonoids. Amongst them ethanol fraction showing shinoda test positive for polar...

13 February 2021 8,263 3 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

How we can dissolve highly insoluble compounds in solvent such as DMSO, WATER, MeOH etc.?

We have tried all the aforementioned solvent with heating. So please share your thoughts how can we soluble my compounds.

14 May 2020 4,208 21 View

What is the difference between Power Transformer Fault Current And Sub Station Fault Level?

What is the reason of Transformer Fault Current is always lower then System fault level?

17 March 2020 2,854 3 View

Is there any method for the isolation of Taphrina maculans causing turmeric Leaf blotch?

Also mention media, pH, temperature and other conditions required for the isolation.

19 October 2019 1,505 2 View

What is the average weight of hypothalamus in Rat?

Hi all, please help me with this question. Thanks a lot in advance!

18 April 2019 4,305 3 View

What things should be taken care of, while performing immunohistochemistry of phospho proteins?

I am doing IHC for normal proteins, but there must be a different protocol to treat phospho proteins. Please, enlighten me with that. Thanks!

02 August 2017 7,414 2 View

How to use cell counting plugin in Image J?

I need to count nerve cells in the tissue stained with Cresyl violet. Please provide me guide to make us of Image J software for cell counting. I have cell counter plugin in Image, but I don't...

01 August 2017 9,887 2 View

Which material gives higher buckling load CFRP or Aluminium?

I am currently conducting research on the buckling of thin plates and simulating a range of materials in abaqus. It seems according to my results that Aluminium gives a higher buckling load...

26 July 2017 9,697 4 View

What boundary conditions do you recommend for a simply supported thin plate under compression?

I am using Abaqus to simulate, the initial boundary conditions which i have tried, is giving me a error of 32% with the theoretical solution. And this is for buckling analysis.

06 June 2017 6,663 3 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

What are the causes of hyphae granulation?

Greetings, The examination of the interaction zone between Fusarium oxysporum and Pseudomonas by light microscope revealed clear hyphae without septa and the presence of granulations (vesicles),...

05 February 2021 5,523 4 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

Calculation of dilution factor for fumonisin extracted rice cultured with fusarium species?

hello I would like to ask for help regarding calculation of dilution factor to get the amount of mycotoxin per gram of sample. the extraction protocol as fellow: -ground 5 g of sample with 25 mL...

29 November 2020 1,831 15 View

How many references sequences should use for a phylogenetic tree construction?

I have identified fungus isolates from the fruit surface by SSU +ITS+ LSU regions, such as : 1) Fusarium kyushuense , 2) Fusarium proliferatum ;3) Aspergillus aculeatus ;4) Aspergillus europaeus...

15 July 2020 5,573 2 View

How old is the "fungal isolate" most suitable for pathogenicity testing on the surface of fruits?

I have different fungus isolates such as; 1) Fusarium kyushuense , 2) Fusarium proliferatum ;3) Aspergillus aculeatus ;4) Aspergillus europaeus ; 5) Aspergillus flavus ; 6) Penicillium...

01 July 2020 6,889 3 View

Is there an alternative for the enzyme mixture Driselase (from Basidomycetes) for Fusarium protoplast generation?

Dear all, is somebody familiar with an alternative to the enzyme mixture Driselase (from Basidomycetes) for Fusarium fujikuroi and/ or Fusarium graminearum protoplast generation? Were are using...

16 June 2020 5,865 2 View

Appearance of crossing point signal even in water during qPCR. Help?

We have been running qPCR to detect Fusarium in wheat grains. Suddenly out of nowhere, we kept getting crossing point signal in the blank (PCR water). We have done the following: ran with only...

02 March 2020 3,388 1 View

Appearance of crossing point signal even in water during qPCR. Help?

We have been running qPCR to detect Fusarium in wheat grains. Suddenly out of nowhere, we kept getting crossing point signal in the blank (PCR water). We have done the following: ran with only...

01 March 2020 1,408 3 View

Does the total RNA contaminated by DNA?

Dear Scientists, I performed RNA extraction of Fusarium oxysporum using TriZol method. I got the gel electrophoresis result. I suspect contimation with DNA. How can I interprete of this result?

31 December 2019 5,801 4 View