Below is the article title shared for you to get the no of Secondary Metabolites present in Olive bark.
Focus on Olea europaea L. pruning by‐products: extraction techniques, biological activity, and phytochemical profile
Generally this analysis done by HPLC and further by LCMS or GCMS.
If you are developing a kit for secondary metabolite of Olive bark on color change basis that is also good. for this you need the derivatizing agent and the corresponding color change after derivatizing the secondary metabolite.
e. g Ninhydrin used as derivatizing reagent for compounds like amines or amino acids.
Olive bark is a term used to describe the outer layer of the bark from an olive tree. It is composed of layers of thick, dark-colored cork cells, which protect the inner bark from weather and pests. Olive bark is harvested for use in various products, including homeopathic medicines, cosmetics, and dyes.