Unable to Form Hydrogel from Decellularized Amniotic Membrane ECM: What Might I Be Missing?

12 October 2023 0 9K Report

Hello everyone,

I've been working on producing a hydrogel from the extracellular matrix (ECM) derived from the human placental amniotic membrane. I've followed a process where I first decellularized the amniotic membrane and then digested it using pepsin. I've tried to adjust the pH and salt ion concentration of the digest at low temperatures. However, no matter what I do, I cannot get it to gel. I would greatly appreciate any insights or suggestions.

Here's a brief overview of my procedure:

Decellularization of the amniotic membrane: Ensuring a thorough decellularization, possibly considering enhancement with combined chemical and physical methods.

Pepsin Digestion: Using an appropriate concentration and ensuring pepsin activity. I've tried varying concentrations.

Adjustment of pH and Salt Ion Concentration: This has been a key focus, trying different levels to find an optimal setting.

Temperature: I've kept the digest at a low temperature throughout the process.

Matrix Concentration: I've tried varying concentrations to see if that might be affecting gelation.

Are there critical aspects or conditions that I might be overlooking or need to optimize? Any guidance would be greatly appreciated.

Thank you in advance!

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