Hello everyone
I'm trying to perform HLHKA test
but I have some questions preparing input files
that is, I'm confused about the calculation of D value.
in the manual, D is described as pairwise number of between-species differences (from a random sequencefrom each species)
so should I just simply chose one sample from my ingroup and compared the number of site difference to the outgroup as the D value?
thanks for any helps!
Pei-Wei, Sun
Department of Life Science, NTNU, Taiwan