I found a medium to differentiate between Bacillus species using : Peptone, Meat extract, D-Mannitol, Sodium chloride, Chromogenic mixture, Phenol red, Agar.
And I want to understand what they meant by chromogenic mixture. To understand how to prepare it
Any help please ?!
Hi Baraa,
Kindly look for the attached link. You may get the idea
Chapter Pathogenic Bacillus species
Chapter Pathogen update: Bacillus species
Hi,
The chromogenic mixture is mainly composed of two substances: 6-chloro-3-indolyl-ß-D-galacto-pyranoside and 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide.
Nowres Jaber I think that the polymyxin pyruvat egg yolk agar only for the differentiation of Bacillus cereus and I can not find bakara agar on google ! what is this medium ?@@
Alaa Kareem Niamah could you please explain to me what will this mix stain? will it do the same differentiation as in HiCrome™ Bacillus Agar " The chromogenic mixture present in the medium is cleaved by the enzyme beta-glucosidase found
in B.cereus resulting in the formation of blue colonies. B.thuringiensis also grows as blue/green colonies on this medium as
B.cereus and B.thuringiensis are biochemically identical, however B.cereus shows flat colonies with distinct blue centres,
while B.thuringiensis shows irregular margins. If selective isolation of B.cereus or B.thuringiensis is required aseptically add
polymyxin B (FD003)."
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