What is the cheapest way to measure luminescence?

More Ghazaleh Chegini's questions See All

What is the contact time(in industry) for powdered activated carbon in wastewater treatment?

I want to study the behavior of different powdered activated carbons (PAC) in removing of contaminants from wastewater. However, I am not sure what would be the ideal contact time for industries...

06 July 2019 5,027 10 View

Could anyone give me some references on the effective range of CO2/biochar ratio to study?

I am calculating from Boudouard reaction the amount of CO2 needed for the activation but I am assuming 100% carbon which is not true and a high flowrate might be problematic for bio-char...

10 November 2018 7,017 0 View

What is the fastest way to analyse (plant) nutrients (Na, K, Mg, p, Fe, Mn, Zn) in water?

So I've been reading about infrared spectroscopy to detect the nutrients in the liquid. I have no clue how it works. I know for FT-IR you have, basically, a reference wavelength that you associate...

06 July 2018 9,659 8 View

How to calculate environmental stability and equivalent ageing of the accelerated biochar?

I have some oxidized biochar samples using several methods from literature. Was wondering how I can correspond the carbon content of the oxidized biochar to calculate the residence time (years) of...

01 January 1970 1,955 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Assistance with MTT Assay Data ?

I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...

26 July 2024 7,511 3 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

Arizaldo Castro

This methods review paper might give you some ideas: Article Measurement of Bacterial Bioluminescence Intensity and Spect...

Badreddine Mechri

Dear Ghazaleh,

I hope that these papers will be useful. Best Luck.

http://2015.igem.org/wiki/images/b/b1/TU_Eindhoven_Protocols_Measuring_Bioluminescense_and_fluorescence.pdf

http://www.comm-tec.com/Library/Tutorials/CTD/Chemiluminescence%20and%20Bioluminescence%20Measurements%20.pdf

Ghazaleh Chegini

Thank you.