What is the cause of the differences between glass microscope slides in retaining cells?

03 March 2018 1 7K Report

I try to optimize a micronucleus assay in cell lines (suspension cells). I switched to frosted slides from non-frosted ones, and I seems that cells do not attach properly to them. My cells are Carnoy-fixed cells, previously I had no problem with this. Is this a common thing? What could be the cause and how could I circumvent this problem?

Thanks :)

Rita Lozsa

Thanks for the answer. Unfortunately we have no cytospin. Is there a way to make the slide more sticky? I know poly-lysine coating, but it is quite expensive. Maybe with some chemicals?

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