Rita Lozsa

5 Questions 13 Answers 0 Followers

Questions related from Rita Lozsa

What is the cause of the differences between glass microscope slides in retaining cells?

I try to optimize a micronucleus assay in cell lines (suspension cells). I switched to frosted slides from non-frosted ones, and I seems that cells do not attach properly to them. My cells are...

03 March 2018 6,701 1 View

Can I use a PCR mix for probe assay with intercalating dye for real-time PCR?

I would like to know the opinion of real-time experts about the following: Do you think it is correct and acceptable for publication, if I optimize a real-time assay using a buffer developed for...

03 March 2015 1,867 6 View

Are sealable tubes necessary for CsCl gradient centrifugation?

What is the reason that most protocols use sealable ultracentrifuge tubes for CsCl gradients? Is it just for safety because of nucleic acid stain or is there another reason?

11 November 2014 2,540 3 View

Do you have any experience on the stability of soil DNA?

I don't have any experience of soil DNA, but I had to make some extractions. I used ZR Soil Microbe DNA MiniPrep kit from Zymo Research. For the first sight, almost all samples were OK, only some...

10 October 2014 684 7 View

Should uploaded sequences in NCBI database also include flanking primer sequences?

Universal primers could cover differences in the flanking region, so officially, should primers be in the sequence or should they be omitted?

02 February 2013 2,315 3 View