Hello

We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the fragment of interest. However, when we purify it with ExoSAP-IT and use it for PCR amplification, when electrophoresis is performed there is a nonspecific banding and the expected product is hardly observed. Can you help me know what is happening?

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