I want to get an estimate of carbon weight in an 'average' lipid, protein, and carbohydrate. Please help
specifically, how to modify the serial communication protocols
26 July 2023 6,330 0 View
Many methods call for reconstituting experimental samples in methanol/ water, but diluting the standards with some sort of plasma (typically blank bovine plasma). Why not dilute the standards in...
28 June 2023 2,437 4 View
I am interested in any articles that have heuristics in the title.
25 April 2023 2,473 2 View
This question seeks to explore the latest developments in tissue regeneration and stem cell therapies specifically for the treatment of combat-related injuries, with a focus on how these...
17 April 2023 1,380 1 View
Hello, I am trying to replicate the knockout of the cytoplasmic catalase production gene CTT1 as done in this paper: Improving carotenoids production in yeast via adaptive labor... . I use the...
28 April 2022 425 1 View
I'm interested in pedagogical, staff development, equity, and collection development practices that further decolonization.
17 November 2021 6,369 4 View
We have developed a method to record methane release in the breath and flatus of animals in real time. Is anyone interested in doing this in their experimental systems?
25 October 2021 8,389 0 View
Samples were homogenized with a mortar and pestle and then passed through a syringe but at the first centrifuge step the sample is getting stuck - we can't see anything on the membrane and only...
13 October 2021 8,786 3 View
Our lab uses Penstrep for assays that contain Adenovirus; however, PenStrep usage for Adeno-Associated Virus+ Adenovirus assays is not permitted. It's important to mention that assays that allow...
13 September 2021 1,897 0 View
I have an O shaped water bath with a square cross section that I am circulating with an immersion circulator. How do I measure the flow rate at a given point in the bath? Can I use an anemometer...
15 August 2021 3,777 3 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...
25 July 2024 637 5 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?
18 July 2024 9,831 0 View
I have conducted a survey on the "effect of vacuum drying conditions on carbohydrate content of angelica.S". The result show that, when increasing the vacuum degree, the carbohydrate content first...
13 July 2024 592 1 View
What is the protocol for storing phospholipids in chloroform solution? Should I divide the contents of a bottle into aliquots to prevent melting each time I take it out for an experiment from...
27 June 2024 2,138 2 View
I'm currently working on preparing a 10 mL liposome solution containing HSPC, mPEG2000-DSPE, and cholesterol at a molar ratio of 55/40/5. can anybody advise on the best way to calculate the weight...
26 June 2024 1,135 0 View
Hi everyone It is my first time working with this technology, so someone here can give me your experience my objective is to study different nucleolar lipids and their interaction with...
25 June 2024 7,351 0 View
The line appears always in the penultimate run. We try to decrease the volume of the solvent system, wich is composed of butanol:acetic acid:water (2:1:1) (76 mL) but the line remains. Below I...
24 June 2024 9,829 3 View
Hello, do you know what the phospholipids of the membrane of rat smooth muscle aortas are composed of? outer membrane-inner membrane
19 June 2024 221 0 View