We have developed a method to record methane release in the breath and flatus of animals in real time. Is anyone interested in doing this in their experimental systems?
Good Evening, My name is David Marshall and I am Doctor of Management Student at Colorado Technical University Online. I just recently received approval from the IRB to start data collection on...
08 February 2021 346 7 View
I am working on a FACS experiment with mouse neural tissue. I want to preserve all cell types as much as possible and the zinc fixation papers I have read use mechanical dissociation which can...
21 January 2021 4,430 1 View
I am separating neuron populations from mouse brains using FACS. I am trying to find a fixation technique for intracellular antigen staining and FACS that will allow long term storage afterwards...
21 January 2021 9,722 3 View
I have been trying to remove the vitreous humour from baby rat eyes (post natal day 15) and have been still having a lot of interference in my IHC staining. Does anyone have a good protocol to...
20 January 2020 4,634 1 View
I have a silicone part that will come in contact with food. It will need to pass FDA tests for hexane extraction of silicone residues. Is there a method I can use to remove uncured material and...
09 December 2019 4,246 3 View
I am attempting to mesh a complicated design (~80,000 faces) for a microchannel heat sink, as pictured, and I would appreciate some advice. I have tried a range of different mesh controls...
08 October 2019 5,610 7 View
I am Trying to use Infusion cloning to prepare a mutant construct. I have been told by a student that used it before that I need 20ng/ul of linearised plasmid to proceed with the following...
04 April 2019 646 10 View
Specifically memory when writing memoir.
03 April 2018 3,172 2 View
I'm looking to mitigate in vitro phospholipid degradation over extended periods of time.
20 February 2018 8,740 3 View
It strikes me that likelihood-based approaches are prone to the similar dichotomous decisions as traditional hypothesis testing. E.g. In the environmental sciences we now seem to accept that...
14 October 2017 1,264 8 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?
02 March 2021 557 2 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...
24 February 2021 7,470 7 View
Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...
24 February 2021 9,105 2 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View