1. To germinate the seeds on petriplate and use the meristems of the root and dip into the nanoparticles solution for 0,1, 2 hrs ?
Or
2.First dip the seeds in nanoparticles solution for desired period and then allow it to germinate and then fix the root meristems for cytology assay?
I work with nanos in fish rather than seeds, but I saw that no one had replied to your question so I thought I would send a few suggestions to you. I have seen several talks on seed germination and growth with nanoparticle exposure and both of the methods you listed have been done (see publications below). The best method may be dependent on the properties of your nanoparticles (type, size, coating, etc.), species of interest (water plant, terrestrial, etc.), and environmental relevance (how would your species encounter the nanos in the environment?).
Good luck!
- Melissa
Article More than the Ions: The Effects of Silver Nanoparticles on L...
Article Effects of Silver Nanoparticle Exposure on Germination and E...
Article Phytotoxicity of soluble graphitic nanofibers to model plant species
Dear Melissa Chernick,
Thanks for adding the answer along with reference papers.
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