Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

How to remove straight line from mosaic Landsat image?

I have used mosaic to new raster tool to join two aerial image from Landsat 8 of different path. However, raster files had color variation and after mosaic I got a straight line running from...

22 December 2020 4,640 4 View

Can anyone please give me some good suggestions for my undergraduate mechanical engineering thesis?

Our group need to start giving topics on which one should we focus on regarding non renewable resources. I've looking these past 2months but nothing comes to mind.

27 November 2020 4,414 3 View

Can someone help with variable selection for logistic regression while considering missing data?

I have a data set with quite a lot of missing values, more in some variables than others. I performed bivariate analyses and I want to select variables for multivariate analysis. I understand that...

25 October 2020 3,613 3 View

To find maximum compressive stress in concrete using hognested model numerical?

In a reinforced concrete beam depth id 640mm.Depth of neutral axis is 0.43363d.Determine max compressive stress in concrete using hognested model when strain in steel reaches0.00284.Solve

20 October 2020 3,303 2 View

What analysis will be best suited to analyze my likert type questionnaire with both likert item and likert scale type Data in SPSS?

https://forms.gle/METKBvvq6n6eFMLb6 Above is a link to my questionnaire. It has three objectives to evaluate. 1) From Page 2-6 the questionnaire ask the stress factors that effects the...

05 September 2020 3,960 3 View

How long time should we wait for editor decision on a manuscript?

Some editors keep a paper for a long time, more than 6 months or a year, without a decision. So I ask how a paper takes a long time like that for the review process and What the best way to...

13 August 2020 5,797 3 View

Can antibodies that come in serum format be used directly in ChIP, or should they be purified before use?

Hello everyone, I am performing ChIP in plant tissues. I bought a ChIP grade antibody from a company but it came in serum format (unpurified). My question is, do I need to purify it first or do I...

10 August 2020 4,545 2 View

Interpreting MICOM Results in SMART PLS?

Hi, What if in the results of MICOM, one out of six constructs does not have compositional invariance (step2). however, for all constructs the difference in means and log variance is...

08 April 2020 1,545 3 View

Is partial inhibition zone of bacteria considered as inhibition?

I work with the antimicrobial activity of natural products. I found that on Mueller Hinton agar plate one of the natural product is producing circular partial inhibition zone around it. Will that...

28 January 2020 2,364 11 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View