When using AAVs to transfect neurons with DREADDs in the past, I've used a glass micropipette to inject the virus into the brain. However, this method only covers
You can inject bigger volume and in faster infusion rate. With that, i get around 3-4 mm area spread of my AAV.. Of course , it also depends on ur viral titer too
If you inject neonatal mice using a 33 Gauge Hamilton, you can deliver 5 microlitres of vector, or more, in a single injection over only a few seconds. There is no microglial activation or glial scarring. Check out Article Intravenous administration of AAV2/9 to the fetal and neonat...
or https://www.nature.com/articles/s41591-018-0106-7 for some of the therapeutic stuff we've done. You get even better distribution by fetal injection but that is a lot harder, technically
sorry, forgot to mention the important bit - neonatal (or fetal) injection allows you to express throughout the entire brain (including cortex). But as Waleed mentioned, putting in the highest titre you can will help. Also we've found the hSynapsin promoter to be awesomely strong: https://academic.oup.com/hmg/advance-article/doi/10.1093/hmg/ddz317/5698692
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