What is the best way to take biological material and virally infect it out of the bio safety lab level 3 or 4, for proteomics analysis?

25 March 2014 1 2K Report

Is there a specific protocol for this? Using formaldehyde or trisolve will destroy the samples for mass spectrometry analysis.

Stéphane Roche

If you perform the lysis of your sample in the safety lab after you could get it out. To do 2D gel, I do the lysis using urea, thiourea and CHAPS. This buffer would inhibit all viral activity.

After lysis, you must check the safety before get out.

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