How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Has any research on translation investigated the phenomenon of bilingual authors who produce versions of their books in both their languages?

Can the second-language version, strictly speaking, be considered a translation of the first-language version or is it merely a somewhat looser interpretation? How do such authors approach the...

10 December 2020 5,092 25 View

How to distinguish CA1 pyramidal cell vs. interneuron using voltage-clamp?

I am doing voltage-clamp experiments in CA1 pyramidal cells (mEPSCs; AMPA-NMDA ratio; LTP; etc) from acute hippocampal slices in mice. I want to make sure I am only recording from excitatory...

29 October 2020 1,098 2 View

Increase Oleophilicity while decreasing hydrophilicity on silicon surfaces?

Hello RG community, currently I am trying to reach these goals on silicon surfaces for a microfludic project. I have used silanes like APTES in the past in order to get my si-surface hydrophobic...

22 March 2020 2,925 3 View

How could one improve the magnitude of t-statistics of a construct?

Hello All, I am not even sure if this is possible, but I have used this dataset for several publication -albeit with different constructs-and have not seen such low t-values. For example, I have a...

10 March 2020 1,164 3 View

How do I calculate the Mean and Standard Deviation for a set of items (i.e., that form a construct) in SPSS?

Hello All, I have been asked to supply the Mean and Standard Deviation for my latent constructs. Is there a straightforward way of doing it in SPSS? I am actually modeling in SmartPLS, but not...

04 March 2020 1,597 4 View

Any strategies for quantification and purification of a red pigment with no peaks in the visible region which is only soluble in water?

We have isolated a red pigment producing bacteria. We harvested the crude pigment by removing the cells by centrifugation. We tried to find the absorption maxima (lambda max) of the pigment using...

19 January 2020 1,331 2 View

How to Create Small Graphs for Interaction Effects?

Hello All, I have been able to calculate all the interaction effects for a study involving the Big Five. One of the interaction effects I would like to graph (among others) is the relationship...

30 December 2019 6,640 3 View

Can you have stop codon right before a RBS (Shine-Dalgaro) site?

I am designing a primer for a gBlock and I don't want to the poly-histag. So naturally I am adding a stop codon in my primer, but then I need a new RBS site. So my question is can I have a stop...

28 July 2019 1,675 2 View

XML haar cascade for red blood cells and/or echinocytes ?

Hello community, we currently working on an automatic algorithm for erytrocyte detection in stereo-microscopy images for cell counting. Additionally we want to determine the percentile amount of...

23 October 2018 3,262 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View