Hello All,
I recently harvested some of my tissue samples and stored them in RNA Later solution in the -80C fridge. Is that the correct way to store or will it cause RNA degradation? Please provide your insight.
If you say muscle sample alone, you did not immerse it in any RNA protecting solutions?
Hello
No problem , but should be removed the RNAlater reagent after overnight immersion, then store your samples at −80℃ .
Good Luck
Hi,
I have compared RNA which was freshly extracted with that of RNA stored at -80°C for over a month in RNA later reagent and both of them had comparable concentration as well as purity. So, I don't think there will be any problem as long as you store it in -80°C.
Thank you all for your valuable insights. I did get a good RNA yield from these tissue samples.
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