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Questions related from Achintyan Gangadharan
I am doing a pilot study to assess the cytotoxic abilities of different T lymphocyte populations when cocultured with B16 Ova In Vitro. I have seen different articles labeling their...
05 May 2019 3,029 7 View
I am still trying to figure out on how to gate lymphocytes population on FLowJo. Attached are 5 plots where all samples are murine spleen and lymph nodes cultured in vitro. 1) My 1st question is...
01 January 2019 6,368 4 View
Hello All, I will soon start to use HPLC and LCMS for 2 different studies. I have an animal tissue and a cell culture to be used for both the studies. The question that I have is what is the...
09 September 2017 706 4 View
My lab is utilizing a H2S instrument (Metrohm) for liquid hydrocarbon testing and I wasn't able to find a reference solution for it. We use the reference as a Quality Control (as with any other...
07 July 2017 9,051 3 View
Hello All, I recently harvested some of my tissue samples and stored them in RNA Later solution in the -80C fridge. Is that the correct way to store or will it cause RNA degradation? Please...
07 July 2016 5,890 4 View
I have been trying to isolate total RNA from healthy rat sciatic nerve but the concentration has always been very low in the range from 50-65ng/ul. I have tried different weights of the nerve,...
03 March 2016 4,815 1 View
I am currently working on an RT-PCR experiment designing different set of genes for running on a 96 well plate. When contacted several companies, some said they adopt intron spanning primer assay...
01 January 2016 3,615 2 View
As my experiment involves RNA isolation, I just want to quickly check the total RNA integrity on a gel and confirm it on a UV-spectrophotometer. For that purpose, is a non denaturing agarose gel...
01 January 2016 9,425 9 View
