How is CTL Assay carried out with Annexin v and PI?

I am doing a pilot study to assess the cytotoxic abilities of different T lymphocyte populations when cocultured with B16 Ova In Vitro. I have seen different articles labeling their...

04 May 2019 2,951 7 View

How to gate lymphocytes from murine spleens and lymph nodes on FlowJo?

I am still trying to figure out on how to gate lymphocytes population on FLowJo. Attached are 5 plots where all samples are murine spleen and lymph nodes cultured in vitro. 1) My 1st question is...

31 December 2018 6,264 4 View

Ways to prepare biological samples for HPLC and LCMS?

Hello All, I will soon start to use HPLC and LCMS for 2 different studies. I have an animal tissue and a cell culture to be used for both the studies. The question that I have is what is the...

08 September 2017 621 4 View

What is the best way to store tissues at -80C which are immersed in RNA Later reagent ?

06 July 2016 5,834 4 View

How to isolate RNA from rat sciatic nerve?

I have been trying to isolate total RNA from healthy rat sciatic nerve but the concentration has always been very low in the range from 50-65ng/ul. I have tried different weights of the nerve,...

02 March 2016 4,761 1 View

How does an intron spanning and exon-exon primer assay work to avoid gDNA contamination?

I am currently working on an RT-PCR experiment designing different set of genes for running on a 96 well plate. When contacted several companies, some said they adopt intron spanning primer assay...

31 December 2015 3,454 2 View

Is a non denaturing agarose gel a good method for visualizing RNA integrity?

As my experiment involves RNA isolation, I just want to quickly check the total RNA integrity on a gel and confirm it on a UV-spectrophotometer. For that purpose, is a non denaturing agarose gel...

31 December 2015 9,374 9 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View