I cut the bands of interest after loading the proteins to the gel but I would like to keep them for a further MS analysis. Should I leave them with water? acetic acid solution? some specific buffer?
Thanks in advance
I suggest storing them in the buffer that is used for the next step in the procedure.
Andrea Guedez
I would cut the bands out and then either freeze them and store at -20
Agreed with both.
If you use water, protein will release to water so it's better to use run sds page buffer or de staining buffer as a cheaper ones.
But I didn't use any buffers as mentioned by Krueger.
Regards
Another option is to equilibrate the stained gel with 5% glycerol and then dry it o/n between two sheets of cellulose acetate (you can get the sheets and the frame from BioRad, but the former cheaper in household stores, they are used for covering jars of homemade jam). Then you can cut the band from the dried gel and store it at -80 °C in a suitable vessel, w/o solvent. This would avoid protein degradation as much as possible.
The dried gels also keep very well in a lab book, btw.
I would also propose to dry gels/sections for storage. Later you can restore them using your runing/equilibration buffer.
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