I dissolved RNA in TE buffer, which I made in DEPC treated water.
yes,you can store it only for few days in DEPC but the best way to store is to reverse transcribed the RNA as cDNA then store it in -20 or -80 degree centigrade,the stability is major question during storage.
I kept RNA in DEPC water for 5-6 month. It was working very well. I can't convert all into cDNA, because I have to do qRT-PCR for different genes.
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
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Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
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Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View