I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to achieve the sensitivity diagram of a sensor in CST?

I wanna design a sensor in CST 2021 for the first time and I don't know how to achieve the sensitivity diagram of the sensor in CST. Could you plz help me to do that? I appreciate your response...

26 January 2021 6,864 1 View

What kinds of potential genes could be expressed in frogs exposed to chytrid fungus in different environmental conditions ?

I need an applicable molecular biomarker like expressing the genes in frogs who expose to chytrid fungus in different environmental stressors. Indeed, what kinds of genes I should notice in frog's...

25 January 2021 7,718 2 View

How to select the most non-correlated samples within a dateset?

Dear researchers How to select the most non-correlated samples within a dateset? Considering that the samples are time-series of length l within m different time-series. To more clarify the...

13 January 2021 741 6 View

DSC test- Glass transition temperature of the electrospun PA66 nanofibers?

Hello Everyone, I did several DSC tests on the electrospun PA66 nanofibers to obtain the glass transition temperature. According to the material datasheet, the Tg point of PA66, as cast, is around...

13 December 2020 1,506 2 View

How to create a sag and swell detection in the DVR applications?

I have created a three-phase cascaded h-bridge inverter at Simulink with the SHE-PWM technique, and I want to test it in the DVR applications. How can I design a controller for that in the DVR...

13 December 2020 735 3 View

Degenerated semiconductor (Burstein Moss effect) with unchanged band-gap?

Is it possible to produce a degenerate semiconductor using a dopant agent that results in increasing the electrical conductivity, but the optical band-gap remains unchanged? In such a situation...

11 December 2020 8,338 13 View

Which statistic software can give me a relationship for multivariate function?

Hi friend I have some data and they influence on each others and I draw their graph in excel but excel can't give me a relationship for multivariate function. the regression of these data in excel...

29 November 2020 9,371 9 View

A paper may be rejected because of several reasons?

In my case, I have received rejection after seven months from submission to journal without reason or editor comments (decline). Moreover, without being reviewed by an expert scientist reviewer,...

29 October 2020 5,437 4 View

Do scalar-tensor-vector gravity theories violate the Bekenstein-Hawking area law to compute the entropy of black holes?

I am looking for the thermodynamics of the cosmological black hole in scalar-tensor-vector gravity theories. I know how should I write and find the thermodynamical quantities and equations for...

26 September 2020 6,383 3 View

How to select substrate concentration to determine the inhibitor behavior as competitive or non-competitive?

I already calculated the IC50 value of the inhibitor against the protease enzyme. Now I need to find this inhibitor competitive non-competitive. I decided to perform the Lineweaver Burk plot, but...

21 January 2021 8,378 3 View

Best purification method for recombinant protein in BL21?

Hi I need to purify a recombinant protein (IL6 21kDa). It's been produced in E.coli BL21 with a T7 expression vector, but it does not have a tag as yet. I was thinking of adding a his-tag and...

09 December 2020 2,200 8 View

How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

Can TEV protease cleave between glutamine and methionine efficiently?

Based on this paper The P1 ' specificity of tobacco etch virus protease ,TEV protease can cleave between the Gln and several amino-acids (besides Gly/Ser) with acceptable efficiency in its...

26 November 2020 5,987 4 View

What is the best method to check interaction between a drug and a protein ?

Hi, I would like to know the best method to check the interaction between a proteine (cruzipain, cysteine protease) and a drug. It has been purposed to our project to evaluate protein-drug...

30 October 2020 7,209 3 View

How to purify a recombinant protein from Ecoli to reach a cell culture purpose?

05 October 2020 502 7 View

Protease activity assay?

Hello, I would like to get help! I want to measure the protease activity of pancrease powder. I prepared 10mg/ml stock solution from the pancrease powder and I used 100ul from that and I added...

22 June 2020 1,609 3 View

Why my protein has lower molecular weight that expected?

Hi, I am trying to make a kind of recombinant protein (Flag-tagged) from HEK293T cells. But when I check the cell lysate by WB, the molecular weight is lower that expected. The molecular weight...

09 June 2020 6,414 13 View

No protein expression in Pichia, any ideas?

I introduced a heterologous gene using pPIC 9 plasmid into KM71 strain and confirmed the presence of the target gene by PCR. The colonies (6) I am checking growing well on Minimal growth media...

25 May 2020 1,611 9 View

In which environments would you bioprospect microorganisms with proteolytic activity?

I need to isolate microorganisms that can produce proteases, in which environments can I find them Justify answer Thank you

27 April 2020 7,669 1 View