I want to do transcriptomic study on 5dpf zebrafish larvae. The larvae will be exposed to toxic to see the differential gene expression between control and exposed zebrafish. What is the best way(s) to euthanize this larvae. Im afraid if using tricaine will affect the transcriptome and if using cold water, the larvae will experience hypoxic condition..please help
Thank you very much Elizabeth E LeClair
..really appreciate it
MS-222 is an excellent method for larvae and it will not affect expression levels if you choose the proper dose and time
Thank you for the suggestion Manuel Manchado ..At first im thinking of using cold water..but now i will consider using MS-222.
I collect the larvae in an eppendorf tube, and then I place the tube on ice for a couple of minutes. The larvae will go to the bottom of the tube, so you can easily remove the water and add your extraction buffer
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