I want to look at the genotoxicity of my coating material to mammalian cells. From whatever I have seen so far, the tail moment is commonly used. What else is considered standard?
Thank you!
For Comet assay? OTM is the best way to represent data. You can also use RAPD, to detect genomic damage. Also Southern using probe for repeat elements like LINE-1 can also be done.
Best,
Somnath
Kedar, you could also express the results % tail DNA which is a good indication of how badly damaged your cells are.
Although there are several endpoints for the Comet assay, the maojority of authors recommend the use of the DNA content in the tail (% tail DNA) to assess DNA damage.
hi, many comet indexes used in comet analysis(about 15 to 16 index). But the most important indexes is five index includes: comet length, comet height, tail length, % DNA in tail and tail moment. In More Articles, % DNA in tail and tail moment used.
the best, simple and free software for comet assay analysis is comet score program.
Comet length and tail length are cmmonly used as end points .They can be easily measured by any relevant software. We used comet score 15
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