Dear All

I am extracting RNA from urinary cell pellet with mirvana Kit from ABI.

These are our results from the best round of extraction :

260/280 : 1.86

260/230 : 1.02

cons : 88.6

I need help to find my pitfalls or maybe the best way to manipulate the protocol to remove alcohol. Below is the protocol we use for extraction.

I appreciate any suggestion .

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