Has anybody used mirvana kit to extract RNA from urinary cell pellets?

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Mehri Barabadi

Dear Dr Rane

Thank a lot for your kind answer and your great suggestions.

As you mentioned,our major problem is low cell input in urine .It affects our yield and at the end of process the best cons has been 88 till now. But about 260/230 ratio I thought maybe we need some change in the steps related to removing phenol.

we changed washing with washing buffer 2/3 from 2 times with 500 into 3 times with 400 .

I ll increase the centrifuge time to see if 260/230 ratio gets better.

And a question :

why should we use elution solution at 95 degree?I mean what exactly happens in this temperature?

Yes exactly .

We have financial limitations so I guess the idea of changing the protocol is more possible :)