Dear ALY,

You can follow the attached manual for quantification and identification of bacteria. I have used same manual in my research to identify bacteria from fermented products. You can check my publications also.

Regards

Md Faisal

Dear Faisal

Many thanks

Dear Aly,

You have not indicated the type of fermented food product you are interested in. You will need to identify both bacteria and fungi (moulds and yeasts).

Bergey's Manual of Systematic Bacteriology will give you a comprehensive overview of the bacterial population and nature. Also check Moreiro et al. 2001.

Isolation and identification of yeasts and filamentous fungi from yogurts in brazil. Brazilian Journal of Microbiology and Fungi families picture gallery; identification guide - First Nature .

All the best!

Philippa

If you need quantify viable microorganism you should make plate count with serial dilutions. You can use MRS agar for isolate Lactic bacteria, WL agar with chloranphenicol for yeast and preenrichment media for acetic bacteria (with manitol, ethanol and yeast extract); TSA for mesophilic aerobic bacteria; YPD agar for yeast and mould. For identify you can use biochemical test (conventional or kits like a API Biomerieux) with morphological test; or you can use PCR with sequentiantion or PCR with restriction enzymes.

Bergey's is a good book for bacterioloy and The Yeasts, A Taxonomic Study is very nice for yeast.

Dear Alvaro

Thank for your help

Hello everyone, I am also working on the microbial biodiversity of a fermented product, I have targeted several flora among them: Yeasts, molds and lactic acid bacteria,

My questions are:

1/ For isolation of lactic acid bacteria i used the MRS (incubation in anaerobiosis at 30 ° C for 72 h). I would like to know is the use of this medium only gives a representative results or no?

2/ After isolation:I would like to identify the isolates by the application of molecular techniques but I can't identify all isolates, I have to choose a limited number, my question is on what basis I choose this number of strains?

Hi Rania,

You can search morphological and biochemical difference or technological capacities, and finally identify differents patterns of microorganism.

The media MRS is orientative, some bacterias like oenococcus genus growth better in MLO medium.

Thank you very much dear Álvaro Rodríguez

Cher Prof, vous pouvez utiliser la cytommétrie flux, cela devrait vous permettre à le faire